Home > Sandwich ELISA Kits > Human Canopy FGF Signaling Regulator 2 (CNPY2) ELISA Kit

Product Name:

Human Canopy FGF Signaling Regulator 2 (CNPY2) ELISA Kit

Cat.#:

JL16775

Regulatory Status:

RUO

Alternative:

ZSIG9; Cnpy2; MSAP; TMEM4; Transmembrane Protein 4; MIR-interacting saposin-like protein; Putative secreted protein Zsig9;Canopy 2 Homolog

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.075ng/mL

Specificity:

This kit recognizes Human CNPY2 in samples. No significant cross-reactivity or interference between Human CNPY2 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| CNPY2

Canopy FGF signaling regulator 2 is a protein that is encoded by the CNPY2 gene.Predicted to be active in endoplasmic reticulum. Diseases associated with CNPY2 include Fanconi Anemia, Complementation Group C and Craniosynostosis. It prevents MIR-mediated MRLC ubiquitination and its subsequent proteasomal degradation.It enhances the migration of C6 glioma cells by regulating the phosphorylation of light chains through myosin.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.27	1.62	1.07	0.65	0.44	0.33	0.24	0.08
Corrected OD	2.19	1.54	0.99	0.57	0.36	0.25	0.16	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human CNPY2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	95
Cell Lysates(n=8)	96-105	102

| Linearity

Samples were spiked with high concentrations of Human CNPY2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-94	88-96
1 : 2	Average (%)	91	90
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	98